rabbit anti brd3 Search Results


a302 368a rrid ab 1907251  (Bethyl)
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mouse anti brd3  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology mouse anti brd3

Mouse Anti Brd3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cat 11859 1 ap rrid ab 2065902  (Proteintech)
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brd4 a301 985a100  (Bethyl)
96
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rabbit anti-brd3  (Active Motif)
90
Active Motif rabbit anti-brd3

Rabbit Anti Brd3, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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western blot santa cruz sc 8349 rrid ab 2253533 brd3  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc western blot santa cruz sc 8349 rrid ab 2253533 brd3
KEY RESOURCES TABLE
Western Blot Santa Cruz Sc 8349 Rrid Ab 2253533 Brd3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-brd3  (Thermo Fisher)
90
Thermo Fisher anti-brd3
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Anti Brd3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti brd3  (Bethyl)
91
Bethyl anti brd3
(A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, <t>BRD3,</t> and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).
Anti Brd3, supplied by Bethyl, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti-rabbit igg h&l chain specific peroxidase  (Millipore)
90
Millipore goat anti-rabbit igg h&l chain specific peroxidase
(A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, <t>BRD3,</t> and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).
Goat Anti Rabbit Igg H&L Chain Specific Peroxidase, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brd3  (Bethyl)
92
Bethyl brd3
(A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, <t>BRD3,</t> and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).
Brd3, supplied by Bethyl, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti- -actin mouse mab  (Millipore)
90
Millipore anti- -actin mouse mab
(A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, <t>BRD3,</t> and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).
Anti Actin Mouse Mab, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Molecular Cell

Article Title: Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function

doi: 10.1016/j.molcel.2017.04.027

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-BRD3 , Bethyl Laboratories , Cat#: A302-368A; RRID: AB_1907251.

Techniques: Control, Membrane, Virus, Recombinant, Sample Prep, Flow Cytometry, Western Blot, Microscopy, Plasmid Preparation, Phospho-proteomics, Variant Assay, shRNA, Software, CRISPR

KEY RESOURCES TABLE

Journal: Cancer cell

Article Title: BRD4 inhibition is synthetic lethal with PARP inhibitors through the induction of homologous recombination deficiency

doi: 10.1016/j.ccell.2018.01.019

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BRCA1 (D-9) antibody Santa Cruz sc-6954, RRID:AB_626761 ERK 2 (C-14) antibody Santa Cruz sc-154, RRID:AB_2141292 RAD51 (H-92) antibody for Western blot Santa Cruz sc-8349, RRID:AB_2253533 BRD3 (2088C3a) antibody Santa Cruz sc-81202, RRID:AB_1119692 CtIP (D76F7) Rabbit mAb antibody Cell Signaling Technology #9201S, RRID:AB_10828593 MRE11 (31H4) Rabbit mAb antibody Cell Signaling Technology #4847S, RRID:AB_10693469 53BP1 Antibody Cell Signaling Technology #4937S, RRID:AB_10694558 BRD4 (E2A7X) Rabbit Antibody Cell Signaling Technology #13440S, RRID:AB_2687578 BRD2 (D89B4) Rabbit mAb antibody Cell Signaling Technology #5848S, RRID:AB_10835146 RAD51 (Ab-1) Rabbit pAb antibody for immunofluorescence and IHC Millipore Corp PC130, RRID:AB_2238184 Histone H2A.X, phospho (Ser139) Monoclonal antibody, Unconjugated, Clone jbw301 Millipore Corp 05-636, RRID:AB_309864 RPA32/RPA2 antibody [9H8] Abcam ab2175, RRID:AB_302873 RAD50 antibody [EPR3466(2)] Abcam ab124682, RRID:AB_11000808 NBS1 antibody [Y112] - ChIP Grade Abcam ab32074, RRID:AB_777007 Phospho RPA32 (S4/S8) Antibody Bethyl Laboratories A300-245A, RRID:AB_210547 Phospho RPA32 (S33) Antibody Bethyl Laboratories A300-246A, RRID:AB_2180847 RPA70 antibody [EPR3472] Abcam ab79398, RRID:AB_1603759 Histone H3 antibody - ChIP Grade Abcam ab1791, RRID:AB_302613 H3K27ac-human antibody Abcam ab4729, RRID:AB_2118291 PARP-1 (H-250) antibody Santa Cruz sc-7150, RRID:AB_2160738 Pol II (CTD4H8) antibody Santa Cruz sc-47701, RRID:AB_677353 Histone H3 (mono methyl K4) antibody - ChIP Grade Abcam ab8895, RRID:AB_306847 normal Rabbit IgG Cell Signaling Technology #2729.

Techniques: Western Blot, Immunofluorescence, Recombinant, Staining, Lysis, SYBR Green Assay, Transfection, Plasmid Preparation, In Vitro, In Vivo, Mutagenesis, Chromatin Immunoprecipitation, Fractionation, Cell Culture, Expressing, Inhibition, Software

(A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, BRD3, and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).

Journal: bioRxiv

Article Title: Transcription Machinery Anchors ecDNAs to Mitotic Chromosomes for Segregation

doi: 10.1101/2024.12.31.630921

Figure Lengend Snippet: (A) Heatmap for the mRNA expression level of all human bromodomain-containing proteins in ecDNA cancer cell lines. Data were obtained from public RNAseq. TPM: transcripts per million. (B) Western blot for BRD2, BRD3, and BRD4 in SNU16 cells after dBET6 treatment for 24 hours. (C) Western blot showing BRD4 degradation after dBET6 treatment for 24 hours in COLO320DM cells. (D) Representative MYC ecDNA FISH on 1000 nM dBET6 treated COLO320DM metaphase cells (scale bar: 5 μm). (E) The number of detached MYC ecDNA per COLO320DM metaphase cell after dBET6 treatment (two-sided Wilcoxon test). (F) RNAi knockdown efficiency of BRD2 , BRD3 , and BRD4 quantified using qPCR (two-sided Student’s t-test). (G) Representative MYC and FGFR2 ecDNA FISH in SNU16 metaphase cells after BRD2 , BRD3 , or BRD4 RNAi knockdown (scale bar: 5 μm). (H) The number of detached MYC and FGFR2 ecDNAs per SNU16 metaphase cell after BRD2 , BRD3 , or BRD4 RNAi knockdown (Kruskal-Wallis test with post-hoc Dunn test). (I) Western blot for BRD4 and HA tag in COLO320DM-BRD4::FKBP12 F36V cells after dTAGv1 treatment. (J) Representative MYC ecDNA FISH on COLO320DM-BRD4::FKBP12 F36V metaphase cells after 500 nM dTAGv1 treatment (scale bar: 5 μm). (K) The number of detached MYC ecDNA per COLO320DM-BRD4::FKBP12 F36V metaphase cell after dTAGv1 treatment (two-sided Wilcoxon test).

Article Snippet: The following primary antibodies were diluted in 2% BSA in TBST and incubated with membranes overnight at 4°C: anti-BRD2 1:1,000 (Bethyl Laboratories A302-583A), anti-BRD3 1:1,000 (Bethyl Laboratories A700-069), anti-BRD4 1:1,000 (Bethyl Laboratories A301-985A), anti-SMARCA4 1:1,000 (Cell Signaling Technology 49360S), anti-H3K27ac 1:1,000 (Cell Signaling Technology 8173S), anti-H3K4me3 1:1,000 (Cell Signaling Technology 9751S), anti-RPB1 1:1,000 (Cell Signaling Technology 2629S), anti-phospho-RPB1 Ser2/5 1:1,000 (Cell Signaling Technology 13546S), anti-c-MYC 1:2,000 (Abcam ab32072), anti-HA tag 1:2,000 (Cell Signaling Technology 3724S), and anti-GAPDH 1:10,000 (Proteintech 60004-1-Ig).

Techniques: Expressing, Western Blot, Knockdown